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It is widely distributed in the Earth’s crust existing in several mineral forms such as fluorite (CaF 2), cryolite (Na 3AlF 6), monofluorophosphate (PO 3F 2−), and fluoroapatite (Ca 5(PO 4) 3F). Introductionįluoride is a common groundwater pollutant, mostly from geogenic sources. Thus, it could be used as an attractive alternative for the determination of fluoride from groundwater. The developed spectrophotometric method has been successfully applied for fluoride determinations in groundwater samples. Applicability of the method was investigated by analyzing groundwater samples spiked with fluoride standards, and satisfactory recoveries in the range of 98.18–111.4 were demonstrated. The precision studied in terms of intraday and interday at three concentration levels showed less than 5.4% RSD. The limits of detection (LOD) and quantification (LOQ) of the method were found to be 0.19 and 0.64 mg/L, respectively. The calibration curve, resulting in good linearity ( R 2 = 0.9997), was considered during quantitative analysis of the samples and in the spiking analysis. Evaluation of fluoride levels showed that Beer–Lambert’s law is obeyed in the range of 0.3–5.0 mg/L at 544 nm. Experimental parameters that influence the determination of fluoride including ligand type, kinetics, pH, and ligand-to-metal ratio were assayed. In this study, a spectrophotometric method was developed for the determination of fluoride from groundwater using Eriochrome Black T (EBT) as a spectroscopic reagent. Among the bottlenecks, the use of toxic chemicals and tedious steps in preparing chemicals (e.g., SPADNS method) are to mention a few. In addition, spectroscopic methods are found important to quantify fluoride levels from water however, several factors hinder its easier determination. Potentiometric method of analysis is reliable for the determination of fluoride in various samples. Thus, determination of fluoride is vital to take appropriate measures upon fluoride contamination of water. Fluoride health problem is a great concern worldwide, most often as a result of groundwater intake.